In a bid to find or refine laboratory research models for cancer that better compare with what happens in living people, Johns Hopkins Medicine scientists report they have developed a new computer-based technique showing that human cancer cells grown in culture dishes are the least genetically similar to their human sources. The finding, they say, should help focus more resources on cancer research models such as genetically engineered mice and 3D balls of human tissue known as "tumoroids" to better evaluate human cancer biology and treatments, and the genetic errors responsible for cancer growth and progress.

It may not be a surprise to scientists that cancer cell lines are genetically inferior to other models, but we were surprised that genetically engineered mice and tumoroids performed so very well by comparison. The new technique, dubbed CancerCellNet, uses computer models to compare the RNA sequences of a research model with data from a cancer genome atlas to compare how closely the two sets match up.

The researchers found that, on average, genetically engineered mice and tumoroids have RNA sequences most closely aligned with the genome atlas baseline data in 4 out of every 5 tumor types they tested, including breast, lung and ovarian cancers.

The investigators say their work adds to evidence that cancer cell lines grown in the laboratory have less parity with their human source because of the complex differences between a human cell's natural environment and a laboratory growth environment. "Once you take tumors out of their natural environment, cell lines start to change.

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John
Editorial Assistant
Immunogenetics Open Access